Sensor-Actor Systems to Combat Oral Peri-Implant Infections

植牙体细菌感染的发生率很高。这些会导致炎症，并伴有组织破坏，这可能对患者造成严重后果。在研究项目期间，将开发新的植入系统，允许特定的抗菌活性物质（所谓的效应物）的释放，作为对感染初始阶段的反应。因此，在空间和时间上，抗菌效应剂的释放将与感染或定植耦合。在此项目中，将开发传感器-动作系统，通过传感器检测信号并通过触发器激活动作（信号转换）。行动者随后释放效应器，从而启动干预。为了实现这些发展，第一步是开发合成细胞传感器-行为系统。有了这些系统，原理问题可以与有效的传感器系统及其在体外和小鼠模型中的转换或可能的扩增的要求有关。此外，这些细胞传感器系统可用于放大信号并与非细胞（化学）ACTOR系统耦合。该项目的第一阶段将包括对必要的信号和效应器浓度以及空间条件进行定量研究。这将导致原型的开发，以证明这些策略在小鼠模型中的可行性。获得的见解将为非细胞传感器-行为者系统的发展铺平道路，其中化学传感器与化学效应系统耦合。从长远来看，在口腔多物种生物膜病理生理学分析和新型抗生物膜物质鉴定/表征项目中鉴定的生物标志物和抗生物膜活性物质将被用作已开发的传感器-行为体系统的信号或效应器。合成生物学中关于细胞传感器生产的开创性基础研究，应用聚合物化学开发非细胞传感器和生物膜研究不仅是当前研究领域的重要内容。更多的情况是，将这些知识整合到种植体耦合传感器-效应系统的发展中，可能会导致牙科种植体长期预后的持续改善。除了由此长期降低发病率和牙科治疗费用外，该项目还可对制定预防、诊断和治疗与种植体有关的感染的替代战略，甚至在其他医学领域，作出重大贡献。

Dental implants exhibit a high prevalence of bacterial infections. These lead to inflammation, accompanied by tissue destruction, which can have serious consequences for the patient. During the research project novel implant systems are to be developed that permit specific release of antibacterial active substances (so-called effectors) as a reaction to the initial phase of infection. Thus, the release of the antibacterial effectors will be coupled to the infection or colonization, both spatially and temporally. During this project sensor-actor systems are to be developed that detect the signals by a sensor and activate an actor (signal conversion) through a trigger. The actor then releases effectors and thus initiates intervention. In order to implement these developments, the first step is to develop synthetic CELLULAR SENSOR-ACTOR SYSTEMS. With these systems the issues of principle can be examined related to the requirements for effective sensor systems and their conversion or possibly amplification in vitro and in the mouse model. Moreover, these cellular sensor systems can be used to amplify the signals and be coupled to ACELLULAR (CHEMICAL) ACTOR SYSTEMS. The first phase of the project will include quantitative studies on the necessary concentrations of signals and effectors, as well as the spatial conditions. This is to lead to the development of prototypes that demonstrate the feasibility in principle of these strategies in the mouse model. The insights won are to smooth the way to the development of acellular sensor-actor systems, in which chemical sensors are coupled to chemical effector systems. In the long term, the biomarkers and anti-biofilm active substances identified in the project ANALYSIS OF PATHOPHYSIOLOGY OF ORAL MULTI-SPECIES BIOFILMS AND IDENTIFICATION / CHARACTERISATION OF NOVAL ANTI-BIOFILM SUBSTANCES are to be used as signals or effectors for the developed sensor-actor systems. The pioneering basic research in synthetic biology on the production of cellular sensors, the applied polymer chemistry for the development of acellular sensors and the biofilm research are not only of importance for the immediate area of research. It is more the case that the integration of this knowledge on the development of implant-coupled sensor-effector systems may lead to a consistent improvement in the long-term prognosis for dental implants. Aside from the resulting long-term reduction in morbidity and costs for dental treatment, the project could make an essential contribution to the development of alternative strategies for the prevention, diagnosis and therapy of implant-associated infection, even in other areas of medicine.