Neuronal RNA-binding proteins in mRNA localization

双链RNA结合蛋白（dsRBP）Staufen2（Stau2）对mRNA向极化神经元树突的转运起关键作用。最近，我们已经确定了Stau 2的第一组生理RNA靶点，包括小G蛋白信号传导（Rgs4）mRNA的调节因子，这与神经精神疾病有关。在这个项目中，我们希望了解Stau2如何特异性地结合到Rgs4 mRNA，以及这种转录本的意义是什么。Stau2与RBP Pumilio2（Pum2）一起是哺乳动物神经元中RNA颗粒的一部分。初步证据表明，这两种限制性商业惯例可能在功能和物理上相互作用。此外，Stau2蛋白在Pum2缺陷型神经元和Pum2缺陷型小鼠中均显著下调。本项目的目的是研究Stau2与Rgs4 mRNA结合的机制基础和功能意义，以及Pum2对Stau2介导的RNA结合的贡献。我们的目的是了解Rgs4 mRNA是如何被Stau2特异性识别的，以及这对Rgs4转录物的稳定性和定位有什么影响。

The double-stranded RNA-binding protein (dsRBP) Staufen2 (Stau2) critically contributes to the transport of mRNAs to dendrites of polarized neurons. Recently, we have identified the first set of physiological RNA targets of Stau2, including the regulator of small G protein signaling (Rgs4) mRNA, which is implicated in neuropsychiatric disorders. In this project, we wish to understand how Stau2 binds specifically to Rgs4 mRNA and what the implications for this transcript are. Stau2 together with the RBP Pumilio2 (Pum2) are part of RNA granules in mammalian neurons. Preliminary evidence indicates that both RBPs may functionally and physically interact with each other. Furthermore, Stau2 protein is significantly down-regulated in both Pum2-deficient neurons as well as in Pum2-deficient mice. The goal of this project is to study the mechanistic basis and functional implications of Stau2 binding to Rgs4 mRNA and the contribution of Pum2 to Stau2-mediated RNA binding. Our aim is to understand how Rgs4 mRNA is specifically recognized by Stau2 and what implications this has for Rgs4 transcript stability and localization.