The role of HIF-1 and its regulators p53 and Smyd2 in right ventricular hypertrophy and failure (B04)

本项目旨在通过功能得与失实验探讨缺氧诱导因子-1 （HIF-1）、肿瘤抑制因子p53、骨形态发生蛋白受体II型（BMPR2）和甲基转移酶Smyd2在心脏内皮细胞（cECs）和心肌细胞（CMs）中的作用及其相互作用。在接受肺动脉捆带手术的小鼠中，我们将分析HIF-1α、p53、Bmpr2和Smyd2细胞（cEC、CM）特异性过表达和低表达对早期适应性和晚期非适应性RV重构的影响。最终目标是了解这些蛋白质在RV重塑中的作用，旨在制定新的治疗策略。

This project aims to investigate the role and interplay of Hypoxia Inducible Factor-1 (HIF-1), the tumor suppressor p53, the Bone Morphogenic Protein Receptor type II (BMPR2) and the methyltransferase Smyd2 in cardiac endothelial cells (cECs) and cardiomyocytes (CMs) by gain- and loss-of-function experiments. The impact of cell (cEC, CM)-specific overexpression and knockdown of HIF-1α, p53, Bmpr2 and Smyd2 in early adaptive and late maladaptive RV remodeling will be analyzed in mice undergoing pulmonary artery banding surgery. The ultimate goal is to understand the role of these proteins in RV remodeling aiming to develop novel treatment strategies.