Integrated bioimaging facility for resolving the nanoscale cell biology (iBIOS)

在合作研究中心（细胞微室生理学和动力学）的框架内，在过去的五年里，奥斯纳布尔<e:2>克大学获得了将超分辨率成像应用于活细胞的深厚专业知识。目前，这些先进的成像方法可以通过分散基础设施的既定成像设施获得。在2017年初，这个基础设施将搬迁到新的研究大楼细胞纳米分析中心（CellNanOS），该中心是专门为此目的而设计的，以便为内部和外部用户提供各种高性能显微镜技术的无缝应用。我们与Eric Betzig实验室和Janelia研究园区合作，建立了晶格光片显微镜，使三维成像超越衍射极限。这种尖端的成像方法将被广泛的用户群体所使用，这在德国是独一无二的。此外，我们目前通过电子显微镜建立纳米分析，特别关注三维成像和冷冻电子显微镜。一个主要的重点在于发展相关的方法来连接动态细胞过程和超微结构数据。结合我们在翻译后标记技术方面的广泛专业知识，这些互补的方法为最高空间分辨率的相关成像提供了独特的潜力。为了充分利用这一潜力，我们需要额外的人员来适应这些复杂的显微镜方法的应用，以适应各种生物标本和科学问题。为此，迫切需要开发相关方法和三维成像的定量和全自动处理工具。CellNanOs建筑与生物系的物理连接及其跨学科概念对该项目非常有益，导致了一个集成的生物成像设施，为显微镜技术，样品制备和数据处理以及面向用户的研讨会量身定制。

Within the framework of the collaborative research center (Physiology and Dynamics of Cellular Microcompartments), a profound expertise in applying super-resolution imaging to living cells was acquired at the University of Osnabrück during the past five years. Currently, these advanced imaging methods are accessible via an established imaging facility with a decentralized infrastructure. In the beginning of 2017, this infrastructure will be relocated into the new research building Center of Cellular Nanoanalytics (CellNanOS) which was specifically designed for this purpose in order to ideally provide a seamless application of diverse high performance microscopy techniques for internal and external users. In collaboration with the Eric Betzig laboratory and the Janelia research Campus, we set-up lattice light-sheet microscopy enabling three-dimensional imaging beyond the diffraction limit. This cutting-edge imaging method will to be made accessible to broad user groups being unique in Germany. In addition, we currently establish nanoscopic analyses through electron microscopy with a particular focus on three-dimensional imaging and cryo-electron microscopy. A major emphasis lies on the development of correlative approaches to link dynamic cellular processes with ultrastructural data. In combination with our broad expertise in posttranslational labeling techniques, these complementary approaches provide a unique potential for correlative imaging at highest spatial resolution. In order to take advantage of this potential, we request additional personnel to adapt the application of these complex microscopy methods to various biological specimen and scientific questions. To this end, the development of quantitative and fully automated processing tools for correlative approaches and three-dimensional imaging are highly required. The physical connection of the CellNanOs building to the department of biology and its interdisciplinary concept is considerably beneficial for this project, leading to an integrated bioimaging facility with tailored premises for microscopy techniques, sample preparation and data processing as well as user-oriented workshops.