ERA NANOSCI: Observation and manipulation of individual type I interferon signalling complexes

ERA NANOSCI：单个 I 型干扰素信号复合物的观察和操作

• 批准号: 39502660
• 负责人: Professor Dr. Jacob Piehler
• 金额: --
• 依托单位: Leids Instituut voor Onderzoek in de Natuurkunde
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/39502660?language=en
• 关键词: ERA; NANOSCI; Observation; manipulation; individual

Ligand recognition by transmembrane multi-protein complexes is propagated by y complex interplay of protein interactions and conformational changes. The dynamic nature of these complexes and the transmembrane architecture ha so far impeded unerstanding of signal transduction mechanism on the molecular level. Furthermore, an important role of local microstructures in the plasma membrane has become evifent. We aim to investigate individual type I interferon signalling complexes reconsti-tuted into structured model membranes. Ligand interaction dynamics, conformational changes and their propagation through the membrane will be determided using single-pair fluorescene resonance energy transfer. The energy landscape of the interactions will be probed by single molecule force spectroscopy. Combining biochemical methods, micro-fabrication techniques and single-molecule detection will provide a unique opportunity to comprehensively explore one of the most important biological processes.

跨膜多蛋白质复合物的配体识别通过蛋白质相互作用和构象变化的y复合物相互作用来传播。迄今为止，这些复合物的动态性质和跨膜结构阻碍了在分子水平上理解信号传导机制。此外，局部微结构在质膜中的重要作用已变得明显。我们的目的是调查个人I型干扰素信号复合物reconstit-tuted到结构模型膜。配体相互作用动力学，构象变化及其通过膜的传播将使用单对荧光共振能量转移来确定。相互作用的能量景观将通过单分子力谱来探测。结合生物化学方法，微制造技术和单分子检测将为全面探索最重要的生物过程之一提供独特的机会。