Rapid, ultra-sensitive protein structure elucidation by mass spectrometry

该项目旨在开发一种快速发现与蛋白质上独特位点结合的分子类别的方法。新药物的开发经常被确定新化学实体与蛋白质目标结合的位点所需的时间和资源所延迟。最近的一项发现使我们能够直接从单个质谱中完全表征大的蛋白质序列。该项目旨在利用这一突破，开发一种快速的新方法，利用全蛋白质谱法揭示配体-蛋白质结合位点。这将使分子和蛋白质目标之间相互作用的新位点能够以高灵敏度快速发现。反过来，这将使下一代生物活性分子的有效设计成为可能。

This project seeks to develop a method for rapidly discovering classes of molecules that bind to unique sites on proteins. The development of new pharmaceuticals is frequently delayed by the time and resources required to identify the sites that new chemical entities bind to protein targets. A recent discovery has resulted in the ability to completely characterise large protein sequences directly from single mass spectra. This project aims to leverage this breakthrough by developing a rapid new approach for revealing ligand-protein binding sites using whole-protein mass spectrometry. This would enable novel sites of interactions between molecules and protein targets to be discovered rapidly with high sensitivity. In turn, this would allow the efficient design of next-generation classes of bioactive molecules.