Micro-scale geometrically and metallurgically adapted surface structures of implants for the defined stimulation of the osteogenic differentiation

金属植入物用于骨科应用已经使用了很长时间。迄今为止，通过表面改变工艺（如喷砂或蚀刻）改善骨整合行为仅在实证研究中获得。目前还不完全清楚哪些表面化学和结构可以支持最初的细胞植入反应。这个跨学科研究项目的目的是系统地研究细胞对（冶金改变的）表面反应的具体机制，这些表面受到电子束的定义微观结构，并用填充材料合金化/浸出。特别有趣的是纯钛在ti6al4v衬底上以局部选择性的方式合金化。在此过程中，应在体外定性和定量地研究成骨细胞对微结构的反应，并分析微结构类型与合金的相关性。此外，还应研究如何将已知的结构过程“SurfiSculpt”（通过表面化学成分的同时可变性而扩展）转移到微观尺度。特别是研究了显微结构表面的局部合金成分，从而可以控制人间充质干细胞向骨形成细胞的分化行为。研究结果将是关于金属表面细胞反应的额外知识，以及确定几何和冶金表面修饰对植入物的积极影响。

Metal implants for orthopedic applications have been in use for a long time. Improvements of the osseointegration behavior by surface-changing processes, such as sand blasting or etching, were so far only obtained in empirical studies. It is not yet completely understood which surface chemistry and structure can support the initial cell-implant response. This interdisciplinary research project has the aim to systematically investigate the specific mechanisms of the cellular response to (metallurgically changed) surfaces which were subjected to defined microstructures by the electron beam and alloyed up/leached with filler material. Particularly interesting is the alloying up of pure titanium on Ti6Al4V-substrates in a locally selective manner. In doing so, the response of bone-forming cells on the microstructures shall be investigated in vitro qualitatively and quantitatively and the correlation of the type of microstructuring and alloy shall be analyzed. Further, it shall be investigated how the known structuring process 'SurfiSculpt' - expanded by the simultaneous changeability of the chemical composition of the surface - can be transferred to the micro-scale dimension. Particularly the local alloy composition of the microstructured surface is investigated so that the differentiation behavior of human mesenchymal stem cells to bone-forming cells can be controlled. Research results will be additional knowledge about the cellular response on metal surfaces and the determination of the positive effect of geometrical and metallurgical surface modifications on implants.