The structure in four-dimensions of a mammalian nuclear body

该项目旨在开发一个涉及基因调控许多方面的微米级分子机器的工作模型。哺乳动物细胞核中的小体比大分子复合物大，比细胞器小。结构、分子和细胞生物学的最新发展使我们能够开始解释它们的结构-功能关系。该项目利用了大量被称为副斑的核体结构和功能数据，目的是建立一个结构模型。它的目标是通过结合基因组工程、超分辨率显微镜、蛋白质组学和体外相互作用研究，在细胞周期中跟踪数十种蛋白质和长链非编码RNA。

The project aims to develop a working model of a micron-sized molecular machine implicated in numerous aspects of gene regulation. Bodies in the mammalian cell nucleus are larger than macromolecular complexes and smaller than organelles. Recent developments in structural, molecular and cell biology are allowing us to begin to interpret their structure-function relationships. This project capitalises on a wealth of structural and functional data on nuclear bodies termed paraspeckles with the aim of developing a structural model. It aims to track tens of proteins and long non-coding RNA from paraspeckles as they proceed through the cell cycle, by combining genome engineering, super-resolution microscopy, proteomics and in vitro interaction studies.