Techniques for probing biological media with holographic angular scattering spectroscopy

在生物和医学科学中，巨大的努力花费在使用简单的软件来费力地标记、计数和测量样品的显微镜图像中的细胞和结构上。在许多情况下，我们可以用未染色样品的单次拍摄尺寸图图像来取代这种效率极低的过程。如果可以增加深度选择性，我们可以对动物进行非侵入性测量，从而大大减少它们的使用。我们可以评估厚组织，使早期非侵入性诊断恶性肿瘤原位或肌肉表征肉质或肌营养不良症。这些益处对研究、动物伦理和健康产生影响，并在生命和医学科学以及肉类行业具有商业潜力。

Huge effort in the biological and medical sciences is spent in using simple software to laboriously mark, count and measure cells and structures in microscope images of samples. We could replace this incredibly inefficient process in many instances with single-shot size-map images of unstained samples. If depth selectivity can be added, we could perform non-invasive measurements on animals, making a huge reduction in their usage. We could assess thick tissues enabling early noninvasive diagnosis of malignancy in tumours in situ or muscle characterization for meat quality or muscular dystrophy. These benefits impact on research, animal ethics, and on health, and have commercial potential in life and medical sciences, and the meat industry.