Genomic mining of plant-induced secondary metabolites in symbiotic cyanobacteria

固氮蓝藻的物种Nostoc punctiforme是有吸引力的合作伙伴，并发现在多种共生与不同的植物和真菌。丝状蓝藻是细菌世界中最复杂的生活方式之一，能够分化成四种不同的细胞类型。N. punctiforme揭示了大量的次级代谢物基因簇。大多数簇是孤儿药，即相关代谢物目前未知。N.的MALDI成像研究与Gunnera manicata共生或在共生伙伴Blasia pusilla附近的punctiforme揭示了共生相互作用后次级代谢组的重编程，并表明迄今为止植物中隐藏化合物的上调。本研究的主要目的是：1）鉴定植物中表达上调的代谢产物和生物合成基因簇;在B附近的点状。pusilla 3）评估次级代谢组的重编程是否是宿主和菌株特异性的4）从结构上阐明迄今为止来自N. 5）分析植物次生代谢产物的作用本项目的结果有望为N.点状的此外，我们希望发现蓝藻的全新代谢产物家族，并估计其生物技术潜力。

Nitrogen-fixing cyanobacteria of the species Nostoc punctiforme are attractive partners and found in manifold symbioses with different plants and fungi. The filamentous cyanobacteria feature one of the most sophisticated life styles in the bacterial world and are capable of differentiating into four distinct cell types. Genome sequencing of N. punctiforme has revealed a large number of secondary metabolite gene clusters. Most of the clusters are orphan, i.e. the associated metabolites are currently unknown. MALDI-Imaging studies of N. punctiforme in symbiotic association with Gunnera manicata or in the neighborhood of the symbiotic partner Blasia pusilla have revealed a reprogramming of the secondary metabolome upon symbiotic interactions and indicate an up regulation of so far cryptic compounds in planta. The aims of the current project are1) To identify metabolites and biosynthetic gene clusters up regulated in planta2) To survey the transcriptional reprogramming of N. punctiforme in the neighborhood of B. pusilla3) To assess whether the reprogramming of the secondary metabolome is host- and strain-specific4) To structurally elucidate so far cryptic compounds from N. punctiforme5) To analyze the role of secondary metabolites in plantaThe results of this project are expected to provide new insights into the governing chemistry balancing cellular differentiation and symbiotic interactions of N. punctiforme. Moreover, we hope to uncover entirely new metabolite families for cyanobacteria and to estimate their biotechnological potential.