Deep molecular characterization of eukaryotic microorganisms´ diversity and community composition in forest soils and the canopy region across biomes using a multiple barcoding approach - micDiv

土壤原生生物以其丰富度高、周转快和作为微生物食草动物的重要功能，在陆地食物网中占据着重要地位。然而，在培养和分子方法的方法缺陷仍然强烈限制了知识的原生生物多样性，使大集团仍然几乎未知。因此，天然原生生物群落的结构和分类群特有的生态功能在很大程度上是未知的。我们将使用先进的培养独立的高通量测序方法，使用组特异性引物和逆转录rRNA-转录组学，对温带和热带生物群落中从森林土壤（枯枝落叶层，矿质土壤）到树冠区域（树皮，树叶，枯木，分支叉，节孔，附生植物）的所有生态分区的原生生物多样性进行全面评估。

Soil protists occupy key nodes in terrestrial food webs due to their high abundance, fast turnover and functional importance as microbial grazers. However, methodological drawbacks in both culturing and molecular methods still strongly limit the knowledge of protist diversity, so that large groups remain virtually unknown. Accordingly, the structure of natural protist communities and taxa-specific ecological functions are largely unknown. We will use advanced cultivation-independent high throughput sequencing methods using group-specific primers and reverse rRNA-transcriptomics for a comprehensive assessment of protist diversity across all ecological compartments from forest soils (litter layer, mineral soil) to the canopy region (bark, leaves, dead wood, branch forks, knotholes, epiphytes) in temperate and tropical biomes.