In vivo imaging of liver organogenesis from human liver bud

本研究旨在建立一个可视化系统，以监测活体肝芽的肝器官发生。为此，我们根据人肝干细胞、人脐静脉内皮细胞（HUVEC）和人间充质干细胞（hMSC）的三维共培养，开发了一种肝芽生成方法。接下来，我们利用超高速共焦显微镜系统建立了一个4D成像系统。然后将人肝芽移植到颅窗内，观察可视化的、重建的具有人血管网的人肝组织。我们已经建立了一种成像技术来可视化和分析人体实体器官的形成。

In this study, we aimed to establish a visualization system to monitor liver organogenesis from liver bud in vivo. To this end, we developed a liver bud generation method according to the three-dimensional co-culture of human liver stem cells, human umbilical vein endothelial cells (HUVEC) and human mesenchymal stem cells (hMSC). Next, we established a 4D imaging system using ultra-high-speed confocal microscope system. Then we transplanted the human liver bud into the cranial window and observed visualized and reconstituted human liver tissue with human vascular network. We have established an imaging technology to visualize and analyze the formation of human solid organs.