Development of artificial peptidase domains

尽管具有新型底物特异性和独特催化性能的人工蛋白酶在分析方法和新的治疗方法中具有潜在的用途，但人工蛋白酶结构域的从头创建从未实现过。该计划旨在开发人工小肽酶结构域，催化酰胺键的自裂解。采用翻译起始重编程方法，构建了一个长度为20 ~ 40 aa、多样性为1013的随机肽库。从所得到的肽库中对活性物种进行了体外选择实验，提供了22个新的肽序列，可以表现出所需的自裂解活性。

Although artificial proteases with novel substrate specificities and unique catalytic properties would have potential utility in useful analytical methods and new therapeutic approaches, de novo creation of artificial protease domain has never achieved. This program aims at development of artificial small peptidase domains, which catalyzes self-cleavage of an amide bond.By using the translation initiation reprogramming method, a short (20-40 aa length) random peptide library with a 1013 diversity was prepared. In vitro selection experiments of active species from the resulting peptide library has provided 22 novel peptide sequences that could exhibit the desired self-cleavage activity.