Systematic analysis on the inter-gene regions of the Escherichia coli genome

在本研究中，我们分析了大肠杆菌基因组中长度大于0.5 kb的基因间区域。首先，基于信息学和大肠杆菌基因组数据库的原始分析，我们推断出94个符合本研究的位点。接下来，根据进一步的数据库分析确定的优先级，系统地构建了这些区域的缺失突变体。其中一种突变体被发现在低温下对细胞生长有明显的敏感性。在该区域还发现了一个特定的序列基序，这被认为支持细胞在低温下生长。同时，我们分析了在基因间区域有一簇dna结合位点的datA区域。DnaA是复制启动蛋白，我们在datA中揭示了调控DnaA的功能机制。

In this study, we analyzed the inter-gene regions which have a length of more than 0.5 kb in the Escherichia coli genome. First, based on original analysis using informatics and E. coli genome database, we deduced 94 sites which meet our present study. Next, according to priority determined by further database analysis, we systematically constructed deletion mutants of the regions. One of those mutants was found to have clear sensitivity in cell growth at low temperatures. A specific sequence motif was also found in the region, which was suggested to support cell growth at low temperatures. In parallel, we analyzed the datA region which has a cluster of DnaA-binding sites in an inter-gene region. DnaA is the replication initiator protein and we revealed functional mechanism in datA for regulating DnaA,