Elucidation of the virus propagation-interference mechanism in plant cells and search for suppressing factors of this process

我们以前已经表明，在水稻原生质体中没有观察到TMV的繁殖，当TMV-RNA单独引入它们。 而将TMV和CMV-RNA同时导入水稻原生质体中，则观察到TMV的增殖。 这一观察结果可以从microRNA功能的角度来解释。 一般认为，在病毒感染时，RNA沉默起作用;然而，在一些病毒产物中，抑制RNA沉默的因子在病毒感染时起作用。 因此，在这项研究中，这些因素之一应该是CMV的2b基因产物。 据推测，水稻细胞中的某些因子已被确定与2b蛋白相互作用。

We have shown previously that the propagation of TMV was not observed in rice protoplasts, when TMV-RNA was solely introduced into them. However, TMV propagation was observed, when both TMV-RNA and CMV-RNA were introduced into rice protoplasts. This observation may be interpreted from the viewpoints of microRNA functioning. Generally speaking, it is said that upon virus infection, RNA silencing functions; however, in some of virus products, factors that suppress RNA silencing function upon virus infection. Thus, in this study one of such factors is supposed to be a gene product of 2b of CMV. It is inferred that certain factors in rice cells have been identified that interact with the 2b protein.