Identification of muscle support cells generated during muscledifferentiation

肌生成素诱导肌生成的机制一直不明确。我们成功地从分化成肌细胞c2c12细胞中分离出肌原素阴性细胞，并通过微阵列技术发现这些细胞表达了与肌原素阳性细胞不同的一系列基因。其中一个基因在邻近肌肉区域表达，该基因的敲低显著影响斑马鱼的肌肉发生和增强细胞凋亡，支持该基因和表达该基因的细胞参与肌肉细胞的维持或存活的观点。检查这些细胞是否由肌肉卫星细胞产生。

Mechanisms of myogenesis induced by myogenin have been elusive. Wehave succeeded to isolate myogenin-negative cells from differentiating myoblast C2C12cells, and have found that those ells express sets of genes different from myogeninpositive cells by microarray. One of these genes is expressed in regions adjacent tomuscle, and knockdown of this gene significantly affected myogenesis and enhancedapoptosis in zebrafish, supporting an idea that this gene and cells expressing this geneare involved in the maintenance or survival of muscle cells. Whether these cells aregenerated from muscle satellite cells was examined.