Establishment of a method for synthesizing artificial Shiro of Tricholoma matsutake using split-root system

为了在可控条件下生产松茸的子实体，需要培养松茸菌丝体和菌根的致密体Shiro。在本研究中，我们试图建立一种利用分根系统合成人工Shiro的方法。对苗床水分状况和插穗苗龄进行了考察，确定了红松、黑松插穗的最佳条件。此外，还建立了分根系统接种外生菌根真菌的方法。利用红松分根系统研究氮素从菌根向非菌根的转移是可能的。因此，我们认为本研究建立的菌根系统在研究外生菌根共生方面是非常有效的。

To produce fruiting bodies of Tricholoma matsutake under controlled conditions, it is necessary to develop Shiro which is compact mass of mycelia and mycorrhizae of T. matsutake. In this study, we tried to establish a method for synthesizing artificial Shiro using split-root system. We examined the moisture condition of the seedbed and the age of seedlings to be used for cuttings, and determined the optimal condition for obtaining the cuttings of Japanese red pine and Japanese black pine. In addition, we established the inoculation method of ectomycorrhizal fungi for the split-root system. It was possible to investigate the translocation of nitrogen from mycorrhizal root systems to nonmycorrhizal root systems using the split-root system of Japanese red pine. Therefore, we considered that the sprit-root system established in this study to be very effective in studying the ectomycorrhizal symbiosis.