Isoprenaline-induced cardiac fibrosis in Tric-a-knockout mice

TRIC-A在可兴奋组织中大量表达。在Tric-a-敲除小鼠中，慢性β-肾上腺素能受体刺激（异丙肾上腺素，Iso）显著诱导心脏纤维化。在这项研究中，为了研究潜在的机制，我们专注于心肌细胞死亡。Tric-a基因敲除小鼠血清肌钙蛋白T水平升高，伊文思蓝染色膜通透性增加，表明异丙肾上腺素刺激诱导的心肌细胞坏死。电镜下观察到Iso刺激Tric-a基因敲除小鼠心肌线粒体功能紊乱，提示Tric-a基因敲除小鼠心肌细胞坏死与Iso诱导的心肌纤维化有关。

TRIC-A is abundantly expressed in excitable tissues. Cardiac fibrosis was significantly induced by chronic beta-adrenergic receptor stimulation (Isoproterenol, Iso) in Tric-a-knockout mice. In this study, to investigate the underlying mechanisms, we focused on cardiomyocyte death. Elevated serum troponin T level and increased membrane permeability of evans blue dye which means cardiomyocyte necrosis induced by isoproterenol stimulation were detected in Tric-a-knockout mice. Moreover mitochondrial dysfunction was observed in Iso-stimulated Tric-a-knockout heart under electron microscopic examination.Thus, it is suggested that remarkable cardiomyocyte necrosis is at least in part related to Iso-induced cardiac fibrosis in Tric-a-knockout mice.