Development of a functional diagnostic system for angiosarcoma by surface plasmon resonance

我们应用表面等离子体共振(SPR)技术对血管肉瘤的循环肿瘤细胞(CTC)进行分析。我们可以从1毫升的人外周血中提取多达10个血管肉瘤细胞，与2000个血管肉瘤细胞和抗CD146抗体混合。血管肉瘤细胞和正常血管内皮细胞均表达血管内皮生长因子受体，两种血管肉瘤细胞系中的一种对血管内皮细胞生长因子受体进行磷酸化。然而，只有正常内皮细胞才显示出血管内皮生长因子的SPR信号。这些结果表明，SPR生物传感器可以灵敏、快速地检测血管肉瘤的CTC。

We applied surface plasmon resonance (SPR) for the analysis of circulating tumor cells (CTC) derived from angiosarcoma. We could recover up to 10 angiosarcoma cells from a mixture of 1 ml of human peripheral blood, mixed with 2,000 angiosarcoma cells and anti-CD146 antibodies. Both angiosarcoma cells and normal vascular endothelial cells expressed VEGF receptors on their surface, and one of two angiosarcoma cell lines phosphorylated their EVGF receptors in response to VEGF. However, only normal endothelial cells showed an SPR signal by VEGF. These results suggest that SPR biosensor may detect CTC of angiosarcoma both sensitively and rapidly.