he clarification of the mechanism of postoperative bone resorption using simulated microgravity machine.

在本研究中，我们试图通过模拟微重力机的机械生物学方法来阐明卸载所致的某种程度的骨丢失的机制。分别在对照组(1G)和微重力(CL)组培养小鼠牙龈上皮细胞株GE1。细胞连续培养10d，分别于第0、3、5、7、10d取材，每组取3例。结果表明，CL组对细胞增殖的抑制作用仅为1G组的一半。此外，由于1G组细胞表面平坦，而CL组细胞边界清楚，因此存在形态上的差异。

In this study, we tried to clarify the mechanism of unloading-induced bone loss in some sort by mechano-biological method using simulated microgravity machine. Murine gingival epithelial cell line, GE1, were cultured in both groups ,control (1G) group and micro gravity (CL) group. Cells were cultured continuously for 10 days and sampling were done on days 0, 3, 5, 7, 10, each taking 3 samples from each group. In result, CL group demonstrated inhibition of cell proliferation to half of that of 1G group. Also, morphological differences were suggested since cells in the 1G group were observed flat in uniform surface while cells in the CL group showed distinct cell boundary.