Genomic analysis of the 11q13.3 amplicon navigation microdissection in oral cancer

目的是建立高水平基因组异常口腔癌细胞基因组结构异常的检测方法。我们使用从福尔马林固定石蜡包埋（FFPE）组织中提取的100ng输入DNA来确认CGH微阵列分析的满意数据。此外，我们正在确认令人满意的CGH微阵列分析数据，使用从载玻片中提取的DNA，在免疫组织染色后将载玻片浸泡在二甲苯中去除载玻片上的覆盖层。为了使用更少量的输入DNA检测基因组异常，我们进行了靶向下一代测序。我们从15ng的输入DNA中检测到口腔癌的基因组异常。

The aim is to develop the methods that detect the genomic structure abnormality in oral cancer cell with high-level genomic abnormality. We were confirming the satisfactory data of CGH microarray analysis using extracted 100ng input DNA from a formalin fixation paraffin embedded (FFPE) tissue. Moreover, we were confirming the satisfactory data of CGH microarray analysis using extracted DNA from a sample mounted in slide-glass which coverslip on the slide were removed by soaking the slide in xylene after immunohistostaining. To detect the genomic abnormality using smaller quantity of input DNA, we performed the targeted next-generation sequencing. We detected the genomic abnormality in oral cancer from 15ng input DNA.