The role of novel gasotransmitter hydrogen sulfide on inflammatory response in oral area

最近的多条证据强烈表明，一氧化氮，一氧化碳和硫化氢（H2S）已被称为气体递质工作的各种细胞功能。持续牵张应力诱导小鼠血管内皮细胞系UV-2细胞中胱硫醚-b-合成酶（CBS）和Nox 2 mRNA表达，4 h后CBS和Nox 2 mRNA表达分别增加3倍和1.8倍。 相反，牵张应激不诱导eNOS mRNA的表达。 我们研究了拉伸应力对人成软骨细胞系ATDC 5细胞中各种氧化还原相关基因mRNA表达的影响。 拉伸应激2 ~ 4小时后也诱导Nox 2、iNOS、eNOS和CBS mRNA表达，但不诱导胱硫醚-V-裂解酶表达。 这些证据表明，持续的拉伸胁迫诱导内源H2S的产生。

Multiple lines of recent evidences strongly suggested that nitric monoxide, carbon monoxide, and hydrogen sulfide (H2S) have come to known as the gasotransmitters worked for various cellular functions. Persistent extension stress induce the mRNA expression of cystathionine-b-synthase (CBS) and Nox2 by 3 times and 1.8 times, respectively, after 4 hours in mouse vascular endothelial cell line, UV-2 cell cultured on stress chamber. On the contrary, the extension stress did not induced mRAN expression of eNOS. We examined the effect of the extension stress on the mRNA expression of various redox-related genes in human condroblastic cell line, ATDC5 cell. The extension stress also induced Nox2, iNOS, eNOS, and CBS mRNA expression after 2 to 4 hours, but not cystathionine-v-lyase. These evidences suggested that persistent extension stress induced production of endogenous H2S.