Identification of SNPs on mature micro RNA to analyze origin of miRNA

近年来的研究表明，血清中存在一定量的循环miRNA，它们与AGO 1和AGO 2等蛋白质相关。这种AGO-蛋白结合的miRNA是相当稳定的，并且在从血清样品中提取和逆转录后可通过聚合酶链反应检测。我们试图通过分析血清样品中存在的循环miRNA来分析分泌每种miRNA的器官。为了进行这种分析，我们试图鉴定miRNA中存在的SNP。我们在全人类基因组分析数据库中搜索了这样的SNP，并鉴定了几个候选SNP。虽然我们尝试用数字PCR检测这样的miRNA，但检测少量的miRNA是相当困难的。需要进一步的研究来检测血清样品中如此少量的miRNA。

Recent research made it clear that there are certain amount of circulating miRNA in serum, which present in serum associated with proteins such as AGO1 and AGO2. Such AGO-protein bound miRNA is quite stable and detectable by polymerase chain reaction after extraction from the serum samples and reverse transcription. We attempted to analyze organs which secrete each miRNA by analyzing circulating miRNA that is present in serum samples. To perform this analysis we tried to identify SNPs that is present in miRNA. We searched for such SNP in entire human genome analyzing human genome database and identified several candidate SNPs. Although we attempted to detect such miRNA with digital PCR, detection of small amount of miRNA was quite difficult. Further study is needed to detect such small amount of miRNA in serum samples.