Establishment of plasma membrane domain identity in epithelial cells (26*)

在本项目中，我们重点研究了上皮细胞中磷酸肌苷（PIP）转化酶与细胞粘附机制之间的联系。我们的假设是，最初的粘附模式通过粘附蛋白与PIP转化酶的偶联传播到质膜和细胞皮层的大规模极化中。我们将使用微模式细胞粘附系统、接近标记方法和可逆化学二聚化来操纵PIP水平来检验这一假设。总之，该项目将为我们提供一个定量模型，了解不同的PIP模式是如何在上皮细胞的膜中建立的，以及它在尖基极化过程中的下游作用。

In this project we focus on the connection between phosphoinositide (PIP) converting enzymes with the cell adhesion machinery in epithelial cells. Our hypothesis is that initial adhesion patterns are propagated into large scale polarization of the plasma membrane and the cell cortex via coupling of adhesion proteins to PIP converting enzymes. We will test this hypothesis using a micro-pattern cell adhesion system, proximity labelling approach and a reversible chemical dimerization to manipulate PIP levels. Altogether, this project will provide us with a quantitative model of how differential PIP patterns are established in the membrane of epithelial cells and its downstream role in the apical-basal polarization process.