Riboswitches and small RNAs in Bacilli under different thermal conditions

在不同温度下生长的细菌必须适应这些条件。这已经在蛋白质和脂类中得到了深入的研究。然而，随着最近小RNA和核糖开关在控制细菌基因表达、代谢和生理方面的普遍重要性的发现，似乎很明显，新的RNA元件必须经过优化才能在其生物体所处的温度下工作。芽孢杆菌是一组革兰氏阳性细菌，其代表（枯草芽孢杆菌）属于最了解的生物体。另一方面，这一群体的许多成员生活在相当恶劣的条件下，其中包括低温和高温。因此，我们将识别新的小RNA调控元件，特别强调碳储存调控和亲冷和亲热生物。对于其中一些元素，我们将确定其生物学功能。有了这些信息，我们将开始对不同温度下调节元件的活性进行研究。基于这项研究，我们将进行诱变，以确定负责RNA调节因子适应不同温度的决定因素。

Bacteria that grow at different temperatures have to adapt to these conditions. This has intensively been studied for proteins and lipids. However, with the recent discovery of the general importance of small RNAs and riboswitches in the control of gene expression, metabolism and physiology in bacteria, it seems obvious that the novel RNA elements must be optimized to work at the temperature their organism lives at. The bacilli are a group of Gram-positive bacteria that have a representative (B. subtilis) that belongs to the best-understood organisms. On the other hand, many members of this group live under rather harsh conditions, among them low and high temperatures. We will therefore identify novel small RNA regulatory elements with a special emphasis on carbon storage regulation and the psychrophilic and thermophilic organisms. For some of these elements, we will identify the biological function. With these information at hand, we will then initiate a study on the activity of the regulatory elements at different temperatures. Based on this study, we will perform a mutagenesis to identify the determinants that are responsible for the adaptation of the RNA regulators to different temperatures.