Multiple target regulation by GcvB sRNA

迄今为止，大多数细菌小调控RNA（sRNA）的研究集中在单个靶mRNA的调控上。然而，越来越多的证据表明，sRNA可能通常起着调节多个靶点的作用。该项目旨在充分了解GcvB sRNA的机制和调控，预计在沙门氏菌和其他细菌中有大量细胞靶点。GcvB是一种广泛保守的sRNA，似乎在全球范围内调节氨基酸的摄取和代谢。有证据表明，为了调节多个靶点，GcvB使用多种机制，但这些机制仍然知之甚少。我们对GcvB的研究将帮助我们更好地理解sRNA如何作为翻译抑制因子，以及哪些因子促进多靶点调控。我们还将确定GcvB本身是如何调节的，并解开GcvB介导的调节的生理意义。此外，我们将努力开发基于深度测序和FACS分析的新方法，这些方法将用于SPP 1258中的其他项目。

To date, most studies of bacterial small regulatory RNAs (sRNAs) have focused on the regulation of a single target mRNA. However, there has been increasing evidence that sRNAs might commonly act to regulate multiple targets. This project aims to fully understand the mechanisms and regulations by GcvB sRNA, for which a large number cellular targets have been predicted in Salmonella and other bacteria. GcvB is a widely conserved sRNAs that appears to globally regulate amino acid uptake and metabolism. There is evidence to suggest that, in order to regulate multiple targets, GcvB uses a variety of mechanisms, but these remain poorly understood. Our study of GcvB will help us better understand how sRNAs act as translational repressors, and which factors facilitate multiple target regulation. We will also determine how GcvB itself is regulated, and unravel the physiological meaning of GcvB-mediated regulation. In addition, we will endeavour to develop new methodology based on deep sequencing and FACS analysis that will be of use for other projects in SPP1258.