Characterization of microbe-microbe, microbe-plant, and plant-plant interactions in the phyllosphere and their influence on microbiome function

SPP DECRyPT有助于阐明植物免疫系统在调节微生物组及其对植物的服务中的作用。这个DECRyPT项目研究植物系统免疫和叶际微生物组之间的相互作用。我们发现，诱导系统抗性（ISR）触发的拟南芥根与有益微生物的相互作用，通过植物-微生物-微生物的相互作用改变了叶微生物群的组成。这些相互作用导致了具有植物生长促进特性的新微生物群的招募。在DECRyPT第II阶段的工作中，我们旨在表征我们在假单胞菌和At-L-Sphere黄杆菌Leaf82之间观察到的微生物-微生物相互作用的分子机制，这导致Leaf82招募到A。拟南芥叶和植物的生长促进。此外，我们的目标是研究相同的细菌和额外的植物-微生物相互作用对大麦（大麦）叶际微生物组的影响。在大麦中，我们将用有益生物的合成群落（SynComs）触发ISR，并研究病原体平脐蠕孢（Bipolaris sorokiniana）的调节作用。我们还将应用非生物胁迫，其总体目标是确定叶际微生物组在应对多重胁迫时的变化。在这样做时，我们遵循的工作假设是，与非生物和有益的和/或致病的生物刺激相关的强大的微生物群变化，可能会加强植物的广谱胁迫耐受性。通过研究叶片微生物组动态对植物-植物相互作用的响应，我们将进一步评估植物之间的长距离相互作用是否稳定了叶际微生物组，利用植物和植物群落更好地承受病原体压力。相关的微生物群功能将在SynCom实验中进行测试。植物生长参数和植物和微生物群的基因表达谱的平行分析将提供微生物组动态对植物健康和健康的影响的见解。总之，这些实验的结果将提供对微生物-微生物，微生物-植物和植物-植物相互作用的见解，以及它们在招募和传播[植物健身促进]微生物群功能到叶圈中的作用。

The SPP DECRyPT contributes to the elucidation of the role of the plant immune system in regulating microbiomes and their services to plants. This DECRyPT project investigates the reciprocal interactions between plant systemic immunity and the phyllosphere microbiome. We found that induced systemic resistance (ISR) triggered by interactions of Arabidopsis thaliana roots with beneficial microbes changes the composition of the leaf microbiota through plant-microbe-microbe interactions. These interactions resulted in the recruitment of new microbiota with plant growth-promoting properties to the leaf. In the work proposed for DECRyPT Phase-II we aim to characterize the molecular mechanisms underlying the microbe-microbe interactions we observed between Pseudomonas simiae and At-L-Sphere Flavobakterium Leaf82, which led to Leaf82 recruitment to A. thaliana leaves and growth promotion of the plants. Additionally, we aim to study the effects of the same bacteria and of additional plant-microbe interactions on the phyllosphere microbiome of Hordeum vulgare (barley). In barley, we will trigger ISR with synthetic communities (SynComs) of beneficial organisms, and study the modulating effects of the pathogen Bipolaris sorokiniana. We will also apply abiotic stress with the overarching aim of identifying phyllosphere microbiome changes in response to multiple stresses. In doing so, we follow the working hypothesis that robust microbiota changes that are associated with abiotic and beneficial and/or pathogenic biotic stimuli, likely fortify broad-spectrum stress tolerance in plants. By studying leaf microbiome dynamics in response to plant-plant interactions, we will further assess if long-distance interactions between plants stabilize the phyllosphere microbiome, harnessing plants and plant communities to better withstand pathogen pressure. The associated microbiota functions will be tested in SynCom experiments. Parallel analyses of plant growth parameters and of the gene expression profiles of both plants and microbiota will provide insights into the effects of microbiome dynamics on plant fitness and health. Together, the results of these experiments will provide insights into microbe-microbe, microbe-plant, and plant-plant interactions, and their role in recruiting and propagating [plant-fitness-promoting] microbiota functions to the phyllosphere.