Development of new ultra micro analytical system based on the acridine fluorometry

以1:9 -吖啶基马来酰亚胺（NAM）测定SH、SeH和SO_2，以2:9-溴甲基吖啶（9-BrMA）测定RCOOH。(1)提出了一种新的NAM合成方法。产量大大提高了。(2)建立了一种新的NAM与SeH的荧光反应。结合- se - se - to - SeH的电反应，设计了新的硒半胱氨酸荧光测定方法。(3)采用NAM荧光法对蛋氨酸转化为同型半胱氨酸后的酶解进行分析。(4)采用新型高效液相色谱试剂9-BrMa对羧酸进行标记。还设计了水解法来确定脂肪的羧酸组成。

Two fluorometry based on 1:N(9-Acridinyl)maleimide (NAM) for SH,SeH and SO_2 and 2:9-Bromomethylacridine (9-BrMA) for RCOOH were developed.(1) A new synthetic method for NAM was developed. The yield was greatly improved.(2) A new fluorometric reaction of NAM and SeH was developed. Electrical reaction of -Se-Se- to SeH was combined new dluorometry of seleno cysteins was designed.(3) NAM fluotometry was used in enzymatic analysis of methionine after convertion to homocysteind.(4) A new fruorometric reagent 9-BrMa was used to label carboxylic acid on high performance liquid chromatography. The hydrolysis was also designed to determine the carboxylic acid composition of fat.