Research on positron emitting 62-zinc and 62-copper radiopharmaceuticals for Nuclear Medicine.

62-锌和62-铜在核医学中是非常适用和适合发射放射性核素的正电子；从62-锌的母体制造62-铜发电机也是可能的。本工作充分利用这些金属的固有特性，开展了正电子发射放射性药物和62-铜发生器的研制工作，具体内容如下：1)在62-锌放射性药物的基础上进行胰腺功能和肿瘤诊断。在小鼠和狗的实验中，收集的结果显示，如果以乙二胺-N，N‘-二乙酸酯的62-锌螯合物的形式注射，62-锌在胰腺中具有高度特异性的生物分布；组织分析显示，62-锌与羧肽酶A和B结合；在促胰液素和CCK-PZ的激素刺激下，通过胰管的62-锌排泄增加；胰腺成像研究显示这种62-锌动员的有效性，演示…进一步说明了62-锌螯合物作为外分泌功能诊断剂的适用性；此外，对荷瘤小鼠的筛查显示，它可能用于原发癌的诊断。2)寻找62-铜双功能螯合剂的开发。在以~(99m)Tm双功能络合剂二硫代氨基硫脲为金属配位的基础上，合成了以不同活性胺基和脂肪酸为母体分子的各种62-铜螯合物。它们的62-铜螯合物的稳定性和生物分布与保留的母体分子功能反映在特定的心肌和胰腺蓄积中，这表明它们在功能诊断中的适用性。此外，以葡萄糖为母体分子的双功能络合剂也获得了有价值的数据。3)制造62铜发生器的实验方法。筛选了柱吸附62-锌的原理及其子体62-铜的分离和洗脱条件，为开发新型的62-铜发生器奠定了基础。较少

The 62-zinc and 62-copper are highly applicable and suitable positron emitting radionuclides in Nuclear Medicine; manufacturing of a 62-copper generator, from its 62-zinc parent is as well plausible. In this work, making the best use of intrinsic characteristics of each of those metals, the development of positron emitting radiopharmaceuticals and 62-copper generator were carried out, as next described:1) BASIS ON 62-ZINC RADIOPHARMACEUTICAL FOR FUNCTIONAL AND NEOPLASTIC PANCREAS DIAGNOSIS. Gathered results in experimental with mouse and dog, showed a highly specific biodistribution of 62-zinc in the pancreas, if injected as a 62-zinc chelate of ethylenediamine-N,N'-diacetate; the tissue analysis revealed the presence of 62-zinc bound to carboxypeptidase A and B. Under hormonal stimulation of secretin and CCK-PZ, an increased 62-zinc excretion through the pancreatic duct was achieved; validity of this 62-zinc mobilization in the pancreas was visualyzed by pancreas imaging studies, demo … More nstrating the applicability of 62-zinc chelate as an exocrine functional diagnostic agent; moreover, a screening with tumor bearing mouse revealed its plausible use for the diagnosis of primary carcinoma.2) SEARCH FOR THE DEVELOPMENT OF 62-COPPER BIFUNCTIONAL CHELATING AGENT. Based on interesting results achieved with 99m-technetium bifunctional chelating agent of dithiosemicarbazone as the metal coordination site, various of its 62-copper chelate derivatives holding diverse active amine groups and fatty acid as parent molecules were synthesized. Their 62-copper chelate stability and biodistribution with the preserved parent molecule functionality were reflected in the specific myocardial and pancreas accumulation, an indication of their applicability in functional diagnosis. Also, valuable data were pursued with a bifunctional chelating agent of glucose, as the parent molecule.3) EXPERIMENTAL APPROACHES ON THE MANUFACTURING OF A 62-COPPER GENERATOR. Screening of the principle governing the column adsorption of 62-zinc and the condition for the separation and elution of its daughter 62-copper set the basis for the development of a new 62-copper generator. Less