Service Project: Mass spectrometric proteomic and structural proteomics on CRISPR-Cas of archaea and bacteria

基于质谱的蛋白质组学已经发展成为解决细胞生物学分析问题的核心技术，并已将其范围扩展到结构生物学。它可以定性和定量地回答蛋白质群体在不同细胞条件下的状态。特别是，蛋白质修饰可以定量评估，以及蛋白质-蛋白质，蛋白质-核酸和蛋白质-配体相互作用可以在氨基酸水平上解析和阐明。该Z项目概述了为SPP 2141项目提供的质谱服务，该项目旨在解决有关CRISP-Cas系统在古细菌和真细菌中应用的蛋白质组学和结构生物学问题。

Mass spectrometry based proteomics has matured into a central technique to address analytical questions in cell biology and has extended its scope well into structural biology. It can provide answers to the state of protein populations in different cellular conditions both qualitatively and quantitatively. In particular, protein modifications can be assessed quantitatively, as well as protein-protein, protein-nucleic acids, and protein-ligand interactions can be resolved and elucidated at the amino acid level. This Z-project outlines mass spectrometric services provided to the SPP2141 project required to address questions on proteomics and structural biology regarding applications of CRISP-Cas systems in archae and eubacteria.