Serological diagnosis of non-A, non-B hepatitis.

试图建立与非甲、非乙型肝炎相关的抗原抗体测定系统。用EV病毒转化法从恢复期黑猩猩淋巴细胞中制备单克隆抗体。我们在感染非甲、非乙型肝炎病毒的黑猩猩肝脏中发现了相应的抗原，而在感染甲、乙型肝炎病毒的黑猩猩肝脏中从未发现过。然而，编码该抗原的cDNA与体细胞基因组杂交，则该抗原未被病毒基因组编码。然后，我们通过免疫筛选克隆了来自日本献血者的丙型肝炎基因组并进行了测序。日本HCV克隆的序列与Chiron克隆的序列不同。NS5区血液学仅为74%。建立了HCV抗体的EIA检测系统。用PCR法建立了HCV基因组cDNA检测系统。

An attempt was made to establish an antigen antibody assay system associated with non-A, non-B hepatitis. We have established monoclonal antibody from convalescent chimpanzee lymphocytes by EV virus transformation method. We found the corresponding antigen in the chimpanzee liver infected with non-A, non-B hepatitis virus and never found in the liver of the chimpanzees infected hepatitis A and B virus.However, cDNA coding this antigen hybridized with somatic genome, then this antigen was not coded by the viral genome.Then we cloned hepatitis C Genome from Japanese donor blood by immunoscreening and sequenced. Sequence of this Japanese clone of HCV is different from Chiron's clone. Hemology is only 74% in region NS5. We established EIA assay system of the antibody against HCV.And we also established cDNA assay system of HCV genom by PCR method.