Konfokal/Multiphoton/FCS Mikroskop

FAU“细胞生物学部门”的科学家使用共聚焦、多光子和荧光相关光谱(FCS)显微镜的机会有限，目前是严重阻碍研究进展的一个主要瓶颈。这些技术需要1)以最高灵敏度和分辨率对不同类型的活植物细胞和组织中的各种高动态、弱荧光探针进行双通道全光谱共聚焦和多光子成像，以及2)基于a)基于FCS和b)基于焦平面内的多光子激发、光激活或荧光探针的多光子激发、光激活或-转换来在体分析蛋白质动力学和细胞质中的钙离子或cAMP浓度。只有首选的供应商提供了1)满足本提案中所述研究所需的所有规格，以及2)具有极佳性价比的显微镜。基于创新新技术的实施，这是目前任何其他供应商都无法获得的，该显微镜允许1)在两个通道中可选的超分辨率或高速共焦成像，2)也在多光子模式下的光谱成像，以及3)由完全集成的高端FCS模块实现的最先进的FCS实验。所要求的显微镜可用工作时间的20%将被行政地纳入“光学成像中心Erlangen(OICE)”，这样FAU中央成像设施的用户也将获得该机构目前无法获得的技术，包括柔性和光谱多光子显微镜以及FCS。

Limited access of scientists at the FAU “Cell Biology Division” to confocal, multiphoton and FCS (Fluorescence Correlation Spectroscopy) microscopy currently represents a major bottle neck that substantially hinders research progress. These technologies are required 1) for two-channel fully spectral confocal and multiphoton imaging of various highly dynamic, weakly fluorescent probes in different types of living plant cells and tissues at maximal sensitivity and resolution, as well as 2) for in vivo analyses of protein dynamics and of cytoplasmic Ca2+ or cAMP concentrations based a) on FCS and b) on multiphoton excitation, photoactivation or -conversion of fluorescent probes specifically within the focal plane. Only the preferred supplier has offered a microscope that 1) meets all specifications required for the research described in this proposal, and 2) comes at an excellent price/performance ratio. Based on the implementation of innovative new technology, which is currently not available from any other supplier, this microscope allows 1) optional super-resolution or high-speed confocal imaging in two channels, 2) spectral imaging also in the multiphoton mode, and 3) state-of the-art FCS experiments enabled by a fully integrated, high-end FCS module. 20% of the working time available at the requested microscope will be administratively incorporated into the “Optical Imaging Center Erlangen (OICE)”, such that users of this FAU central imaging facility will also gain access to technologies currently unavailable at this institution, which include flexible and spectral multiphoton microscopy as well as FCS.