Confocal laser microscope

共聚焦显微镜是免疫学研究中常见的标准技术，可提供不同细胞群和组织的详细空间分析。现代共聚焦激光扫描显微镜具有多个激光线和快速扫描系统，可以分析固定和活标本中的细胞动力学，相互作用和形态。在不同的免疫学和神经免疫学研究问题中，共聚焦显微镜被用于研究中枢神经系统（CNS）及其他部位的免疫细胞群。用共聚焦显微镜研究中枢神经系统中的免疫细胞可以回答关于细胞群的不同方面，例如神经免疫疾病中细胞募集的详细分析或稳态和疾病期间常驻免疫细胞的行为。该领域的最新进展强调了在体内平衡和疾病期间，中枢系统驻留和相关免疫细胞群中迄今未被探索的异质性，具有高度的复杂性、动态和可塑性。因此，迫切需要在研究所内规划和开发研究项目，以精确地识别具有多种标记的细胞群，而且新的报告模型的开发需要在一个标本内对多个参数进行成像。

Confocal microscopy is a common standard technique in immunological research and offers a detailed spatial analysis of different cell populations and tissues. Modern confocal laser scanning microscopes with multiple laser lines and fast scanning systems allow the analysis of cell dynamics, interactions and morphology in fixed and living specimens. In different immunological and neuroimmunological research questions, confocal microscopy is applied to study the immune cell populations in the central nervous system (CNS) and beyond. Studying immune cells in the CNS with confocal microscopy allows to answer different aspects about the cell populations, such as a detailed analysis of cell recruitment in neuroimmunological disease or the behaviour of resident immune cells during homeostasis and disease. Recent advances in the field highlight a so far unexplored heterogeneity within the CNS-resident and –associated immune cell populations during homeostasis and disease with high complexity, dynamics, and plasticity. Therefore, there is an urgent need for planned and developing research projects within the institute to identify cell populations precisely with multiple markers, but also the development of new reporter models demands the imaging of multiple parameters within one specimen.