Image-Flow-Cytometer

图像流式细胞仪是专门为亚细胞水平上不同荧光染料的共定位而设计的，可以高通量地同时检测细胞形态和特定荧光染料的定位。这为描述和机械地研究微生物群落内的相互作用开辟了全新的可能性，特别是当相互作用涉及相互作用物种之一（宿主-病毒和（远端）共生相互作用）的内化时，以及当物种在形态方面迅速多样化时。所提出的图像流式细胞仪对于我们在微生物群落中生态和进化变化的机制理解方面的工作是必不可少的，特别是对于需要同时记录单个细胞或颗粒的几个特征的特征之间的关系的检测。我们计划利用图像流式细胞术定位宿主细胞亚细胞水平的水生病毒，定位宿主细胞内的共生细胞，以及研究细胞形态与蛋白表达定位之间的相关性。成像流式细胞术的高通量允许以统计稳健的方式进一步记录不同相互作用物种的特征。康斯坦斯大学没有成像流式细胞仪。

The image flow cytometer is specially designed for the co-localization of different fluorescent dyes at the subcellular level and the simultaneous detection of the morphology of cells and localization of specific fluorescent dyes in high throughput. This opens up completely new possibilities for describing and mechanistically investigating the interactions within microbial communities, in particular when the interaction involves internalization of one of the interacting species (host-virus and (endo)symbiotic interactions), as well as when species rapidly diversify in terms of their morphology. The proposed image flow cytometer is indispensable for our work on the mechanistic understanding of the ecological and evolutionary changes within microbial communities, specifically for the detection of relationships between traits which requires the concurrent recording of several traits of individual cells or particles. We plan to use image flow cytometry for the localization of aquatic viruses at the subcellular level of host cells, for the localization of symbiotic cells within host cells and for correlations between cell morphology and localization of protein expression. The high throughput of imaging flow cytometry allows further the characterization of traits of different interacting species be recorded in a statistically robust manner. An imaging flow cytometer is not available at the University of Constance.