Immune System variables in healthy subjects and during systemic inflammation

临床研究单位解决分子病理生理途径控制全身性炎症和器官功能障碍的综合方法在转化项目。除个别项目►P1-10外，项目►Z的目的是将基础科学项目的成果转化为临床环境，以开发治疗全身性炎症疾病患者的新诊断和治疗方法。为此，所有项目都依赖于科学专业知识、研究方法和资源以及临床患者材料和样本的交流，这些都汇集在这个项目中。核心项目►Z在研究集群内提供了一个共同的连接接口，以解决研究网络中各个元素之间对合作和互动的迫切需求。我们提供研究基础设施，以测试来自个别项目的新发现，包括其一般病理生理学相关性和临床转化价值。为此，我们还开发并提供了先进的技术，对患者来源的样品进行深入的功能体外分析。特别是，通过免疫表型，我们提供了一个非常通用和无与伦比的工具，用于系统和标准化的广谱分析非常广泛的白细胞亚群（中性粒细胞，单核细胞，T细胞，B细胞，NK细胞，dc等）在全身性炎症疾病的背景下的表型和功能外观。该方法通过使用基于多路复用的分析系统（Luminex 100/200系统）对可溶性标记物进行并行分析来补充，从而能够同时测量来自单个样品的多达100种不同参数。此外，项目►Z还将扩展其分析能力，包括Isoplexis IsoSpark技术平台，用于在单细胞基础上对免疫细胞分泌组进行专门分析。通过功能离体分析和成像流式细胞术，我们将临床队列中单个项目中获得的数据转移并确认为特定的信号通路和转录因子。CRU342第二资助期的9个独立项目将产生大型原始数据集。它们来源于非常广泛的最新技术，分析如此庞大而复杂的数据集需要深厚的生物信息学知识。我们在项目►Z中捆绑了必要的统计专业知识，解决了多种形式科学数据集比较的固有挑战，改进和开发新的数据分析策略，从而为所有其他项目提供生物统计学支持。

The clinical research unit addresses the molecular pathophysiological pathways governing systemic inflammation and organ dysfunction in an integrated approach within translational projects. In addition to the individual projects ►P1-10, the aim of the project ►Z is to enforce the translation of results from basic science projects into the clinical setting to develop new diagnostic and therapeutic approaches for the treatment of patients suffering from systemic inflammatory diseases. To this end, all projects have relied on the exchange of scientific expertise, research methods and resources and clinical patient material and samples all brought together in this project. The core project ►Z provides a common connecting interface within the research cluster that will address this urgent need for cooperativity and interactions in between the individual elements of the research network. We provide the research infrastructure to test novel findings from the individual projects regarding their general pathophysiological relevance and clinical translational value. To that end we have also developed and offered sophisticated technologies for in-depth functional ex vivo analysis of patient-derived samples. In particular, by immune phenotyping we provide a very versatile and unmatched tool for the systematical and standardized broad-spectrum analysis of the phenotypic and functional appearance of a very wide array of leukocyte subsets (neutrophils, monocytes, T cells, B cells, NK cells, DCs, etc.) in the context of systemic inflammatory disorders. This approach is complemented by the parallel analysis of soluble markers using multiplex-based analysis system (Luminex 100/200 system) to enable the simultaneous measurement of up to 100 different parameters from a single sample. Furthermore, the project ►Z will also extend its analytical capabilities by inclusion of the Isoplexis IsoSpark technology platform for the dedicated analysis of the immune cell secretome on a single cell basis. By using functional ex vivo assays and imaging flow cytometry we transfer and confirm data obtained in individual projects in clinical cohorts down to specific signaling pathways and transcription factors. All 9 individual projects included in the second funding period of the CRU342 will produce large raw datasets. They are derived from a very wide range of state-of-the-art technology and analyzing such large and complex datasets requires profound bioinformatic knowledge. We bundle the essential statistic expertise in the project ►Z, addressing the inherent challenges of comparison of multiform scientific datasets, improve available and develop novel data analysis strategies and thereby providing biostatistics support to all other projects.