ImmunoStroke: From Immune Cells to Stroke RecoveryLinking functional immune profiles and ischemic lesion characteristics in human stroke

第一个资助期的重点是中风病变和中风治疗对脑网络结构的特征性影响，以及外周血免疫细胞的免疫特征和功能特性的中风相关变化。在最近这项工作的基础上，我们现在的目标是增加病变网络映射分析，以评估中风后免疫组变化与结构和功能性脑网络结构改变的相关性。基于我们的数据表明髓样细胞区室发生了实质性变化，在第二个资助期，我们的目标是进一步研究中风后单核细胞的功能变化。我们将专注于不同的单核细胞亚群，并使用流式细胞术，转录组学和SCENITH技术在单细胞水平上评估功能，代谢和嘌呤能信号的变化。

The first funding period focused on the characterizing effects of stroke lesions and stroke treatment on brain network architecture as well as stroke-related changes in the immune signature and functional properties of peripheral blood immune cells. Building on this recent work we now aim to add lesion network mapping analysis to evaluate the association of changes in the immunome after stroke to alterations in structural and functional brain network architecture. Based on our data indicating substantial changes in the myeloid cell compartment, in the second funding period we aim to further investigate the functional changes in monocytes after stroke. We will focus on different monocyte subpopulations and evaluate changes in function, metabolism and purinergic signaling at the single cell level using flow cytometry, transcriptomics and SCENITH technology.