Immune System variables in healthy subjects and during systemic inflammation

健康受试者和全身炎症期间的免疫系统变量

• 批准号: 427775384
• 负责人: Professorin Dr. Luisa Klotz
• 金额: --
• 依托单位: Klinik für Anästhesiologie, operative Intensivmedizin und Schmerztherapie
• 依托单位国家: 德国
• 项目类别: Clinical Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/427775384?language=en
• 关键词: Immune; System; variables; healthy; subjects

The clinical research unit addresses the molecular pathophysiological pathways governing systemic inflammation and organ dysfunction in an integrated approach within translational projects. In addition to the individual projects ►P1-10, the aim of the project ►Z is to enforce the translation of results from basic science projects into the clinical setting to develop new diagnostic and therapeutic approaches for the treatment of patients suffering from systemic inflammatory diseases. To this end, all projects have relied on the exchange of scientific expertise, research methods and resources and clinical patient material and samples all brought together in this project. The core project ►Z provides a common connecting interface within the research cluster that will address this urgent need for cooperativity and interactions in between the individual elements of the research network. We provide the research infrastructure to test novel findings from the individual projects regarding their general pathophysiological relevance and clinical translational value. To that end we have also developed and offered sophisticated technologies for in-depth functional ex vivo analysis of patient-derived samples. In particular, by immune phenotyping we provide a very versatile and unmatched tool for the systematical and standardized broad-spectrum analysis of the phenotypic and functional appearance of a very wide array of leukocyte subsets (neutrophils, monocytes, T cells, B cells, NK cells, DCs, etc.) in the context of systemic inflammatory disorders. This approach is complemented by the parallel analysis of soluble markers using multiplex-based analysis system (Luminex 100/200 system) to enable the simultaneous measurement of up to 100 different parameters from a single sample. Furthermore, the project ►Z will also extend its analytical capabilities by inclusion of the Isoplexis IsoSpark technology platform for the dedicated analysis of the immune cell secretome on a single cell basis. By using functional ex vivo assays and imaging flow cytometry we transfer and confirm data obtained in individual projects in clinical cohorts down to specific signaling pathways and transcription factors. All 9 individual projects included in the second funding period of the CRU342 will produce large raw datasets. They are derived from a very wide range of state-of-the-art technology and analyzing such large and complex datasets requires profound bioinformatic knowledge. We bundle the essential statistic expertise in the project ►Z, addressing the inherent challenges of comparison of multiform scientific datasets, improve available and develop novel data analysis strategies and thereby providing biostatistics support to all other projects.

临床研究单位在翻译项目中以综合方法解决了控制全身炎症和器官功能障碍的分子病理生理学途径。除了个别项目CIPP 1 -10外，项目CIPZ的目的是将基础科学项目的结果转化为临床环境，以开发新的诊断和治疗方法，用于治疗全身炎症性疾病患者。为此，所有项目都依赖于科学专业知识、研究方法和资源以及临床患者材料和样本的交流，所有这些都汇集在这个项目中。核心项目ECOZ在研究集群内提供了一个共同的连接界面，将满足研究网络各个组成部分之间合作和互动的迫切需要。我们提供的研究基础设施，以测试新的发现，从个别项目有关其一般病理生理学的相关性和临床转化价值。为此，我们还开发并提供了先进的技术，用于对患者来源的样品进行深入的功能性离体分析。特别是，通过免疫表型分析，我们提供了一种非常通用和无与伦比的工具，用于对非常广泛的白细胞亚群（中性粒细胞、单核细胞、T细胞、B细胞、NK细胞、DC等）的表型和功能外观进行系统和标准化的广谱分析。在全身性炎症性疾病的情况下。该方法通过使用基于多路复用的分析系统（Luminex 100/200系统）对可溶性标记物进行平行分析来补充，以使得能够从单个样品中同时测量多达100个不同的参数。此外，该项目还将扩展其分析能力，包括Isoplexis IsoSpark技术平台，用于在单细胞基础上专门分析免疫细胞分泌组。通过使用功能性离体测定和成像流式细胞术，我们将临床队列中各个项目中获得的数据转移并确认为特定的信号传导途径和转录因子。CRU 342第二个资助期内的所有9个项目都将产生大型原始数据集。它们来自于非常广泛的最先进的技术，分析如此庞大而复杂的数据集需要深厚的生物信息学知识。我们将基本的统计学专业知识捆绑在项目中，解决了多种形式科学数据集比较的固有挑战，改进了现有的数据分析策略并开发了新的数据分析策略，从而为所有其他项目提供了生物统计学支持。