A Study on Fatigue and Recovery of Masticatory Muscles during Controlled Biting by Tissue Blood Flow Measurement

在使用电解产生氢气的间隙法（电解法）中，通过向组织中插入的电极施加电流来产生氢气。我们通过清除率曲线估计局部血流量。然而，在这种方法中有一个固有的问题，因为测量值根据测量本身的条件而变化。例如，我们称之为电解参数的电解电流强度（Id）和电解电流持续时间（Td）。我们确定了四个电解参数：1)Id=3 _<mu>A, Td=30_ <sX> 2) Id=5 _<mu>A, Td=30_s 3) Id=7 _<mu b> A, Td=30_s 4) Id=10 _<mu>A, Td=50_s。我们研究了在休息和休息-握紧-休息序列中咬肌组织局部血流测量值的可重复性。试验时间为两天，间隔超过30天，结果如下：在静止位置，一天内测量的血流量变化很小，第一天和第二天的血流量变化很大，但参数4除外。在休息-握紧-休息序列中，除parameter4外，一天内和第一天至第二天的测量值变化大于仅在休息位置测量的结果。根据这些数据，我们得出结论，在使用这种电解方法时，必须保持插入电极与周围组织的位置关系。我们还推测，握紧拳头导致了他们之间的变化，差距的程度变得更大。在参数4中，产生的氢气比参数1、2或3多，电极在组织中的位置变化对测量值没有影响。在parameter4中，握拳后测量的血流量大于握拳前其余时间测量的血流量。然而，增幅很小。

In the clearance method using electrolytically generated hydrogen gas (electrolytic method), hydrogen gas is generated by applying current to the inserted electrode in the tissue. We estimate the amount of local blood flow by its clearance curve. However, there is an inherence in this method because measurement values vary according to the conditions under which in the measurements themselves were taken. For example, the electrolytic current intensity (Id) and electrolytic current duration (Td) which we call the electrolytic parameters. We determine four electrolytic parameters : 1) Id=3 _<mu>A, Td=30 _<sX> 2) Id=5 _<mu>A, Td=30_s 3) Id=7 _<mu>A, Td=30_s 4) Id=10 _<mu>A, Td=50_s. We investigated the reproducibility of measured values of local blood flow in masseter muscle tissue both at rest and in a rest-clench-rest sequence. The tests were conducted on two days, more than thirty days apart, with the following results :At rest position, the variation in measured blood flow in the course of one day is minimal, and the variation between the first and second days is large, except parameter4. During rest-clench-rest sequence, the variation in the measured values observed in one day and that between the first and second days is larger than the results measured only in the rest position, with the exception of parameter4.Based on these data, we concluded that when applying this electrolytic method, we must maintain the positional relationship between the inserted electrode and surrounding tissue. We also surmised that clenching caused changes to occur between them, and the degree of disparity became greater. In parameter4, more hydrogen gas was generated than parameters1, 2or3, and changes in the position of electrode in the tissue had no effect on measurement values. In parameter4, measurement blood flow after clench was larger than that measured in the rest before clench. However, the amount of increase was little.