Automatic slide scanning microscope for brightfield and fluorescence microscopy

用荧光显微镜可以很好地确定干细胞中的蛋白质及其分布。有了这个半自动显微镜系统，就有可能在“中产”过程中产生高质量的干细胞和骨髓切片的免疫荧光图像。该系统还允许在“中等吞吐量”过程中记录解剖切片。对干细胞中大量新蛋白质分布的筛选也可以使用这样的中通量方法进行。这种新的系统也是新颖的，除了最先进的不同蛋白质的共同分布的研究之外，由于该设备的全自动操作，可以使用新的屏幕来分析稀有干细胞中复杂的和多个蛋白质的共定位。此外，对于所有实验室成员来说，减少在显微镜前花费的必要时间也是相对于简单的工作场所人体工程学的一个重要特征。固定的细胞用系统作为整个载玻片扫描，并用具有扫描像素分辨率的多通道荧光Z-叠层扫描。所有通道都是以真实的灰度分辨率录制的。根据先前开发的扫描配置文件，图像是全自动的和可重现的。扫描数据直接传输到工作组存储(NAS)。扫描数据的评估在两个工作站上通过被称为“细胞分类器”的定制调整/开发软件的全自动分析而自动和交互地进行。

Proteins and their distribution in stem cells can best be determined with fluorescence microscopy. With this semi-automatic microscopy system it will be possible to produce high-quality immunofluorescence images of stem cells and bone marrow sections in a "mid-throughput" procedure. The system also allows the recording of anatomical sections in the "mid-throughput" procedure. Screening for the distribution of a large number of new proteins in stem cells can also be carried out using such a mid-throughput methods. With such a new system also novel, beyond the state-of-the-art investigations on co-distributions of different proteins and new screens to analyze complex and multiple co-localizations of proteins in rare stem cells are possible due to the fully automatic operation of the device. Furthermore, simply a reduction in the time necessary to spend in front of a microscope is also an important feature with respect to simple workplace ergonomics for all lab members. Fixed cells are scanned with the system as whole slides and with multichannel fluorescence Z-stacks with a scanning pixel resolution. All channels are recorded with a real gray scale resolution. The images are fully automatic and reproducible according to previously developed scan profiles. The scan data is transferred directly to workgroup storage (NAS). The evaluation of the scan data takes place both automatically and interactively on two workstations via fully automatic analyses by means of a custom-adjusted/developed software termed "Cell-Categorizer".