Analytical 5 laser flow cytometer

本应用的目的是获得一种分析型多参数高端流式细胞仪。需要一种能够用多达30个参数来表征干细胞和其他稀有细胞类型的设备。到目前为止，海德堡大学及其医院还没有这样的流式细胞仪，大学校园里也没有其他不属于该大学的科学机构。所描述的科学项目包含非常复杂的抗体面板，不能用分析标准的3-激光机器进行处理。由于待分析样本中的细胞数量非常有限，大型抗体小组需要尽可能多地获得信息。额外的多参数分析可以更好地洞察不同的细胞群体。市场上满足这些需求的机器数量有限。此外，目前还没有足够多的荧光染料商业化来全面使用这些流式细胞仪。

The aim of this application is to acquire an analytical multi-parameter high-end flow cytometer. There is a need for a device to characterize stem cells and other rare cell types with up to 30 parameters. Such a flow cytometer is up to now neither available at the University of Heidelberg and its hospital nor on the university campus hosting additional science institutions not being part of the university. The described scientific projects contain very complex antibody panels, which cannot be processed by analytical standard 3-laser machines. There is a need for big antibody panels to get information as much as possible due to the fact that the cell numbers in the samples to be analyzed are very limited. Additional multi-parameter analysis gives a better insight to diverse cell populations. There is just a limited number of machines on the market fulfilling these needs. Additionally, there are not yet enough fluorescent dyes commercialized to use these flow cytometers at full range.