Heat-treated autograft, basic research and clinical application

在热处理的骨中，骨形态发生蛋白在70 ℃，10分钟的条件下保存最多。在此条件下，新的软骨和骨诱导最大量。对不同热处理时间条件下的同种异体骨肌腱止点进行了组织学和生物化学研究。在70 ℃孵育同种异体移植物的组中观察到新骨形成。移植物插入时的拉伸强度检查显示，表面脱矿物质增加了其强度，达到非热处理组的强度。60-70 ℃热处理10小时保持骨诱导活性，如碱性磷酸酶、1型和2型胶原的mRNA的诱导所示，其大致与植入组织的组织学变化和Ca含量相关。

Among heat-treated bone, bone morphogenegic protein was most preserved in a condition of 70 c, 10 minutes. Under this condition, new cartilge and bone was induced in maximal quantiry. Tendon insertions onto allogeneic bone already treated with several kinds of heat and time condition were studied histologically and biochemically. New bone formation was observed in groups with allografts incubated at 70 c. Tensile strengths examination at graft-insertion revealed surface-demineralization increased their strength up to that of the non-heat-treated group. Heat treatment with 60-70 c for 10 hours preserved bone-inductive activity as indicated by an induction of the mRNAs for alkaline phosphatase, type 1 and type 2 collagens which roughly correlated with the histological changes and Ca content of the implanted tissues.