IDENTIFICATION OF STAINS FROM MEDICO LEGAL ASPECTS

一项研究旨在鉴定来自植物色素的污渍。采用高效液相色谱法（HPLC）对13种常见杂草进行了检测，在100 ~ 200 ug的干燥叶片及其染色液中检测到12种黄酮类化合物。以芦丁和鼠李素为参比标准，根据保留指数不同的黄酮类化合物的色谱图谱进行植物种类的鉴别。将所建立的方法应用于三个犯罪案件。受害者或嫌疑人衣服上的污渍，后来被确定为草渍，以及从另一个人身上取下的干草叶，通过检查来确定犯罪现场。根据黄酮类化合物在高效液相色谱上的特征分布规律对各样品的种类进行了鉴定。从证据材料上清晰地追溯了犯罪现场，证明了该方法在犯罪学应用上的实用性。

A study was designed to identify stains derived from plant pigments. Using high performance liquid chromatography (HPLC), 13 species of common weeds were examined, and 12 flavonoids were detected from as low as 100 to 200 ug of dried leaves and from their stains. Identification of plant species should be made, based on the various chromatographic patterns of the flavonoids with different retention indicies, using rutin and rhamnetin as the reference standards.The established method was applied to three crime cases. Stains on clothing from a victim or a suspect which were later on determined as grass stains, and a blade of dried grass taken from another were examined to identify the sites of crimes. The species of each sample was identified based on the characteristic distribution pattern of flavonoids seen on high pergormance liquid chromatography. The scene of the crime was clearly traced from the evidential material and this method was proved to be useful for the criminological application.