Study of the improvement in the resolution of capillary electrophoresis using affinity interaction

本项目的目的是通过一种新的分离机制，特别是在毛细管中引入亲和相互作用来提高毛细管电泳（CE）的分辨率。为了实现这一目标，对不同材料之间的相互作用进行了电化学和电泳研究。以铜(II)-环己基丁酸酯修饰的碳膏电极具有作为金属配合物的氨基酸和硫辛酸的积累和对它们的电化学敏感检测的功能。糖可以选择性地积累在铜(II)-卟啉修饰的电极上，并进行电化学检测。研究了亲和素- bittin相互作用作为蛋白质与配体相互作用的模型。将标记的生物素与电活性化合物如道诺霉素一起使用，电活性部分的电极响应由于与亲和素的特异性结合而降低。基于标记生物素与生物素之间的竞争反应，说明了生物素电化学分析的发展前景。研究了腐植酸与重金属离子的相互作用。发现腐植酸的络合能力随离子强度的增加而增强。在CE中，与毛细管表面形成的半束的相互作用提高了有机阴离子分离的分辨率。根据酸解离平衡和表面十二烷基钠（SDS）的分布平衡，研究了烟酸衍生物的分离。在低pH（2.8）条件下，13种烟酸衍生物虽然需要较长的分析时间，但分离完全。采用胶束电动色谱法，在20 min内实现了13种烟酸衍生物的完全分离。进一步计算了它们在SDS胶束中的分布系数。结果表明，在毛细管中引入亲和相互作用可以提高CE的分辨率。少

The aim of this project is to improve the resolution of capillary electrophoresis (CE) by means of a new mechanism for separation, especially, the introduction of affinity interaction in a capillary.For the achievement of the object, the interaction between various materials was investigated electrochemically and electrophoretically.The carbon paste electrode modified with copper (II)-cyclohexylbutyrate has a function for the accumulation of amino acids and lipoic acid as a metal complex and for the electrochemical datection of them sensitively.Sugars can be selectively accumutated on the electrode modified with copper (II)-porphyrin and detected electrochemically.The avidin-bitin interaction was investigated as the model of the interaction between protein and the ligand.Using the labeled biotin with electroactive compound such as daunomycin, the electrode response of the electroactive part decreased by the specific binding with avidin.The probability in the development of electrochemi … More cal assay for biotin was shown on the sasic of the competition reaction between the labeled biotin and biotin.The interaction between humic acid and heavy metal ions was also investigated.It was found that the complexing ability of humic acid increased with increasing ionic strength.In CE,the interaction with the hemimicelle formed on the surface of a capillary improved the resolution in the separation of organic anions.The separation of niacin derivatives was investigated on the basis of the acid dissociation equilibrium and the distribution equilibrium into sodium dodecyl surfate (SDS).At low pH (2.8), thirteen niacin derivatives were sepaated completely although long analytical time was required.By micellar electrokinetic chromatography, the complete separation of 13 niacin derivatives was achieved within 20 min.Further, the distribution coefficients of them into SDS micelle were evaluated.It was demonstrated that the introduction of affinity interaction in a capillary would offer the improvement in the resolution of CE. Less