Alveolar macrophage-derived chemotactic factor for airway epithelial cells

本课题题目为“肺泡巨噬细胞衍生的气道上皮细胞趋化因子”。然而，尽管进行了大量的实验，但仍未发现其显著的趋化活性。这个项目是我们“气道上皮细胞及其相关细胞趋化性”这个大项目的分支，所以我们现在报告与这个项目密切相关的肥大细胞趋化性和中性粒细胞趋化性两个重要的研究结果。(1)支气管上皮细胞释放纤维连接蛋白作为肥大细胞的趋化因子。培养牛支气管上皮细胞，获得培养上清培养基。与培养液相比，上清液对大鼠嗜碱性白血病（RBL-2H3）细胞和大鼠腹膜肥大细胞具有明显的趋化活性。利用高效液相色谱（HPLC）对其进行连续三次柱层析纯化，最终鉴定为牛纤维连接蛋白。(2)香烟烟雾刺激培养的牛支气管上皮细胞释放中性粒细胞趋化活性。培养牛支气管上皮细胞，在培养基中加入香烟烟雾水溶性提取物。孵育6小时后收获培养上质条件培养基。与不含烟雾提取物的培养上清相比，含有烟雾提取物的培养上清对人外周血中性粒细胞的趋化活性显著提高。趋化活性似乎是脂氧合酶产物。(发表)

The title of this project is "alveolar macrophage-derived chemotactic factor for airway epithelial cells".However, the significant chemotactic activity has not been identified in spite of the continuous and many experiments after all. The project has been the branch of our big project titled "the airway epithelial cells and the related cell chemotaxis", so we now report our results from two important research for the mastcell chemotaxis and the neutrophil chemotaxis, both of which are closely related to this project.(1)Bronchial epithelial cells release fibronectin as a chemotactic factor for mast cells.We cultured bovine bronchial epithelial cells and obtained the culture supernatant conditioned medium. The supernatant had significantly more chemotactic activity for the rat basophilic leukemia(RBL-2H3) cells and rat peritoneal mast cells compared with the culture medium. The chemotactic activity was then purified by three serial column chromatographies using HPLC,and finally identified as bovine fibronectin.(submitted)(2)Cigarette smoke stimulates release of neutrophil chemotactic activity from cultured bovine bronchial epithelial cells.Bovine bronchial epithelial cells were cultured and the water-soluble extract of cigarette smoke was added into the culture medium. The culture supermatant conditioned medium were harvested after six hours incubation. The smoke extract contained culture supernatant contained significantly more chemotactic activity for human peripheral blood-derived neutrophils compared with the supernatant without smoke extract. The chemotactic activity appeared to be a lipoxygenase product(s).(published)