Immunohistochemical Study on Gene Expression of Oral Precancerous Lesions with Reference to Malignant Transformation

应用c-Fos和c-jun多克隆抗体对49例口腔癌前病变、高、中分化鳞状细胞癌和非肿瘤性上皮组织中c-Fos和c-jun基因的表达和恶变进行了组织化学观察。为了提高这些基因蛋白的可探测性，用高压灭菌器或微波(一种抗原修复方法)处理切片，得到以下结果：1.未用抗原修复方法无法检测到c-Fos或c-jun阳性细胞，但经该方法处理后的多种组织中均可检测到c-Fos或c-jun阳性细胞。高压灭菌组的阳性细胞数和阳性反应强度均高于微波灭菌组。在非肿瘤组织中，c-Fos和c-jun阳性细胞均分布于角化层附近的颗粒层。在癌前病变和癌前病变组织中，c-Fos和c-jun基因蛋白并不总是位于同一层上皮组织中。3.比较c-Fos和c-jun在多种上皮性良、恶性组织中的表达强度和阳性细胞数，发现癌前病变和癌前病变中c-Fos和c-jun的阳性细胞数顺序为：非肿瘤性上皮组织&GT；高分化鳞癌&GT；中分化鳞状细胞癌。尤其是c-jun的表达具有更典型的这种趋势。以上结果表明，c-Fos和c-jun的表达受到抑制，既反映了恶性转化，也反映了恶性程度。

Histochemical observation of c-Fos and c-Jun was carried out by using their polyclonal antibodies in 49 patients with various kinds of oral lesions such as precancerous lesions, well- and moderately-differentiated squamous cell carcinomas and non-tumorous epithelial tissues with reference to these gene expressions and malignant transformation. For elevation of detectability of these gene proteins, the sections were treated by an autoclave or a microwave, that is an antigen retrieval method, and the following results were obtained :1.No positive cells for c-Fos or c-Jun could be detected without the antigen retrieval methods, however, c-Fos-or c-Jun-positive cells were detected in many kinds of tissues after treatment with this methods. More number of positive cells and more intensity of reaction were showed in the sections treated by the autoclave than in those treated by the microwave. However, there was no significant difference about distribution of c-Fos-or c-Jun-positive cells between the tissues treated by autoclave and microwave.2.In the non-tumorous tissues, both of c-Fos-and c-Jun-positive cells distributed in the granular layr adjucent to the cornified layr. In the precancerous and cancerous tissues, these gene proteins did not always located in the same layr of epithelial tissue.3.Intensity of reaction and number of positive cells for c-Fos and c-Jun were compared among many kinds of epithelial benign and malignant tissues, and following orders were observed : non-tumorous epithelial tissues>precancerous lesions>well-differentiated squamous cell carcinomas>moderately-differentiated squamous cell carcinomas. Especially, the expression of c-Jun had these tendency more typically. From the above results, it was considered that the expression of c-Fos and c-Jun was inhibited, reflecting to malignant transformation as well as to malignancy.