Serum alpha_1-microglobulin variations in acute reaction phase

α1-微球蛋白(Alpha1-m)是在肝脏合成的一种30 kDa的小分子糖蛋白，与大多数蛋白质一样。我们研究了外科手术患者和阿尔法链病患者血清这种蛋白的变化。2)临床证明α-微球蛋白是一种阳性的急性时相反应物。术后血清值根据所保留肝脏的合成能力而定。由于其分子量低，很容易从肾脏中清除，这使得到目前为止无法将该蛋白定义为急性相反应物。未来的基础研究正在进行中，以阐明肝脏α1-m反应的确切机制。对于临床意义，该蛋白的总测量可用作肝功能及其恢复的敏感指标。2)阿尔法链-α1-m复合体的结构分析阿尔法链-α1-m复合体的基本结构是Fcalpha和Fcalpha-α1-m复合体的异源二聚体，后者以1：1的摩尔比共价结合。α1-m的血清浓度极低，免疫组织化学检查显示受累组织中未见α1-m阳性定位，但结构异常。3)血清淀粉样蛋白A(SAA)分析的建立在本研究过程中，利用乳胶凝集反应建立了一种精确的SAA测定方法。4)α1-m合成的机制通过肝癌细胞系(CCH4)研究了白介素6对α1-m合成能力的影响，结果表明，上清液中α1-m的值降低，这与临床上的发现相矛盾。

alpha1-microglobulin (alpha1-m) is a low molecular weight glycoportein of 30kDa synthesized at liver as most of proteins are. Serum variations of this protein were investigated both in patients with surgical operation and alpha-chain disease.2) alpha1-microglobulin clinically proved to be a positive acute phase reactant. Serum value was ellvated postoperatively depending on the synthetic capacity of the liver reserved. Since its molecular weight was low, it is easily cleared from kidney, that have so far made it impossible to define this protein as a acute phase reactant. Futrher fundamental study is under way to elucidate precise mechanisms of alpha1-m reaction at the liver.For clinical significance, total measurement of this protein can be useful as a sensitive indicator of liver function and its recovery.2) Structure analysis of an alpha-chain-alpha1-m complex in alpha-chain diseaseBasic structure of alpha-chain-alpha1-m complex is a heterodimer of Fcalpha and Fcalpha-alpha1-m complex, in latter of which alpha1-m covalently binds at 1 : 1 molar ratio. alpha1-m was extremely low in serum concentration as compared with that in alpha-chain.Immunohistochemical examinations have shown no alpha1-m positive localization in involved tissues, but structurally abnormal alpha-chain. A complex form should be synthesized elsewhere in the tissues in the body once alpha-chain is released into circulation from involved lymph nodes.3) Development of a serum amyloid A (SAA) assayIn the process of the present study, a precise assay for SAA was developed using a latex agglutination reaction.4) Mechanisms of alpha1-m synthesisEffects of Interleukin 6 was investigated on synthetic capacity of alpha1-m by a hepatoma cell line (cCH4) to show that the alpha1-m was reduced in its value in the supernatant fluids, which contradicted the clinical findings.