Mechanism of synergistic effect on sesame lignans and tocopherols

三个实验表明，芝麻籽及其木脂素显著地产生高的α-生育酚浓度，并改善低α-生育酚饮食大鼠的维生素E状况1)研究了芝麻籽对大鼠血浆和组织中生育酚浓度的影响，这些大鼠的饮食中分别含有10(低)、50(正常)或250(高)毫克的α-生育酚/千克饲料，加或不加20%芝麻籽。血浆和组织中的α-生育酚浓度与饲料中的α-生育酚含量呈递增趋势。芝麻籽可使饲喂任何剂量的α-生育酚的大鼠血浆和组织中的α-生育酚显著增加。然而，只有在低α-生育酚+芝麻组中才发现γ-生育酚的存在，而在正常或高α-生育酚+芝麻组中没有发现。2)在不含维生素E的对照饲料、低α-生育酚饲料和添加5%、10%和15%芝麻籽的三种低α-生育酚饲料中，检测了红细胞溶血、丙酮酸激酶活性和血浆、肝脏和肾脏中作为维生素E缺乏指标的过氧化产物的变化。这些指数在低α-生育酚饮食中很高，而即使添加5%的芝麻籽也能完全抑制这些指标，并导致血浆和肝脏中α-生育酚浓度显著升高。3)两种含有芝麻木脂素(芝麻酚或芝麻素)和低α-生育酚的日粮进行了测试。结果表明，在实验2中，两组芝麻木脂素的饲喂组与饲喂芝麻籽组的结果相当。这些实验表明，芝麻木脂素提高了饲喂低α生育酚饲料的大鼠的维生素E活性，并使饲喂含有芝麻籽或其木脂素的α-生育酚饲料的大鼠血液和组织中的α-生育酚浓度显著增加。

Three experiments demonstrated that sesame seed and its lignans produce significantly high alpha-tocopherol concentrations and improve vitamin E status in rats fed a low alpha-tocopherol containing diet.1) The effect of sesame seed on tocopherol concentrations in the plasma and tissue was studied in rats fed diets containing 10 (low), 50 (normal), or 250 (high) mg alpha-tocopherol/kg diet with or without 20% sesame seed for 8 weeks. The concentrations of alpha-tocopherol in the plasma and tissue showed a progressive increase paralleling the alpha-tocopherol contents in the diets. Sesame seed caused marked increase of alpha-tocopherol in the plasma and tissue of rats fed any amount of alpha-tocopherol. However, gamma-tocopherol was found only in the low alpha-tocopherol + sesame seed-fed group, and not in the normal or high alpha-tocopherol + sesame seed-fed group.2) Changes in red blood cell hemolysis, pyruvate kinase activity, and peroxides in the plasma, liver and kidney, as indices of vitamin E deficiency, were examined in rats fed five diets : a vitamin E-free control diet, a low alpha-tocopherol diet and three low alpha-tocopherol diets supplemented with 5%, 10%, and 15% sesame seed. These indices were high in the low alpha-tocopherol diet, whereas supplementation with even 5% sesame seed suppressed these indices completely and caused significantly higher concentrations of alpha-tocopherol in the plasma and liver.3) Two diets containing sesame lignan (sesaminol or sesamin) and low alpha-tocopherol were tested.Results in both of the sesame lignan-fed groups were comparable to those observed in the sesame seed-fed groups in Experiment 2. These experiments indicate that sesame seed lignans enhance vitamin E activity in rats fed a low alpha-tocopherol diet and cause a marked increase in alpha-tocopherol concentration in the blood and tissue of rats fed an alpha-tocopherol containing diet with sesame seed or its lignans.