Experimental Research for Regeneration of damaged Liver

1.测定肝切除患者淋巴细胞β_2-肾上腺素受体结合量与动脉血酮体比(AKBR)变化的关系。术后低AKBR组较高AKBR组术后淋巴细胞β2肾上腺素受体最大结合量(Bmax)下降更明显。提示肝切除所产生的手术应激可能影响配体-受体结合能力，AKBR可以反映手术应激的大小。2.体外实验和肝移植患者中细胞外FK结合蛋白12(FKBP12)的作用。FKBP12可抑制免疫抑制剂FK506抑制植物凝集素诱导的人外周血单核细胞增殖反应。在临床上，血浆FKBP12水平的快速升高可能通过抑制FK506.3的免疫抑制活性而参与急性细胞排斥反应的发生和发展。短暂低氧可降低肝细胞内ATP含量，但复氧可恢复细胞内ATP含量的下降。这种低氧可减少可用EGF受体的数量而不影响受体蛋白的表达，并通过EGF受体分子的可逆性改变而抑制EGF诱导的大鼠肝细胞DNA合成。

1.In the hepatectomized patients, binding capacity of lymphocyte beta2-adrenoreceptor was assayd in relation to changes in arterial blood ketone body ratio (AKBR) presenting mitochondrial redox potential. In the low post-operative AKBR group, postoperative maximum biding capacity of lymphocyte beta2-adrenoreceptor (Bmax) decreased more that in the high AKBR group. It was suggested that the surgical stress produced by hepatectomy may influence the ligand-receptor binding capacity, and that AKBR can indicate the magnitude of surgical stress.2.The effects of extracellular FK binding protein 12 (FKBP12) were studied in vitro and in patients received liver transplantation. FKBP12 inhibited the ability of FK506 (immuno-suppressive agent) to suppress phytohem-agglutinin-induced proliferative response of human peripheral blood mono-nuclear cells. Clinically, the rapid increase in plasma level of FKBP12 may contribute to the occurrence and progress of acute cellular rejection, probably due to inhibition of the immunosuppressive activity of FK506.3.The effects of transient non-lethal hypoxia on epidermal growth factor (EGF) -induced DNA synthesis of rat hepatocytes were investigated. Transient hypoxia decreased intracellular ATP content, but reoxygenation restored the decrease in ATP.Such a hypoxia decreased the number of available EGF receptors without affecting the receptor protein expression, and inhibited the EGF-induced DNA aynthesis of rat hepatocytes through reversible changes in the EGF receptor molecule.