Studies of Pathogenicity of Campylobacter jejuni.

1.用一种新研制的培养基，可在25 ℃下显示空肠弯曲菌产生霍乱样肠毒素。空肠分离菌株。发现这种新培养基产生的肠毒素量高于先前报道的用于此目的的两种培养基。霍乱毒素和大肠杆菌不耐热肠毒素（和）的抗血清能中和该毒素引起中国仓鼠卵巢（CHO）细胞形态学改变的活性<LT_h><LT_p>，表明这些毒素之间存在密切的免疫学关系.用细胞毒法测定了C.空肠/大肠杆菌在CHO细胞上的表达也用39个C中的33个证明。空肠和所有8个C.大肠杆菌3.由于C.用纯化的滋贺毒素的抗血清中和空肠/大肠杆菌，我们从志贺菌1和大肠杆菌0157：H7中纯化了滋贺毒素和志贺样毒素，比较其结构.我们建立了一种高灵敏度的ELISA检测细菌毒素以及弯曲杆菌毒素。该酶联免疫吸附试验的灵敏度为：产肠出血性大肠杆菌不耐热肠毒素200 pg/ml，志贺菌1型滋贺毒素60 pg/ml，肠出血性大肠杆菌Vero毒素20 pg/ml。大肠杆菌的热稳定直接溶血素（TDH）为6 pg/ml，霍乱弧菌的霍乱肠毒素（CT）为60 pg/ml。

1. By means of a newly developed medium, cholera-like enterotoxin production by Campylobacter jejuni could be shown in 25 C. jejuni strains isolated from diarrheic cases. This new medium was found to yield a higher amount of enterotoxin than the two previously reported media for this purpose. Neutralization of the activity of the toxin to cause morphological changes of Chinese hamster ovary (CHO) cells by antisera against cholera toxin and heat-labile enterotoxins of Escherichia coli ( <LT_h> and <LT_p> ) was also demonstrated, indicating a close immunological relation of these toxins.2. The cytotoxicity of culture filtrates of C. jejuni/coli on CHO cells was also demonstrated with 33 out of 39 C. jejuni and with all 8 C. coli strains.3. Since the cytotoxic activity of the toxin produced by C. jejuni/coli was neutralized by an antiserum against purified Shiga toxin, we purified Shiga and Shiga-like toxins from Shigella dysenteriae 1 and Escherichia coli 0157:H7 to compare the structures.4. We developed a highly sensitive ELISA to detect another bacterial toxins as well as the toxins of Campyrobacter. Sensitivity of this ELISA was 200 pg/ml for heat-labile enterotoxin of enterotoxigenic Escherichia coli, 60 pg/ml for Shiga toxin of Shigella dysenteriae 1, 20 pg/ml for a Vero toxin of enterohemorragic E. coli, 6 pg/ml for thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus, and 60 pg/ml for cholera enterotoxin (CT) of V. cholerae.