Studies on fungal-parasite and fungal-borne viruses

（<I>）真菌寄生病毒：共筛选出真菌病毒600余株，主要为植物病原真菌103属200种。从35种微生物中检出41种新病毒，6种微生物中检出7种已知病毒，表明真菌病毒广泛存在于这些微生物中。直接负染法是检测真菌病毒最有效的方法。本研究中使用的大多数病毒都是从其宿主中纯化的，并对其进行了形态学和理化性质的检查。由于核酸含量的差异，有些菌株是多组分的。在4个病毒中观察到双壳结构，其中2个病毒在12个顶点处具有刺突，这在分类学上具有重要意义。用抗7种病毒的抗血清对分离到的真菌病毒进行了血清学特性的测定，其中2种病毒在血清学上相互关联。细胞内附件 ...更多信息 研究了各病毒的生物学特性。通常，病毒被认为是细胞质或液泡中的扩散和聚集或晶体阵列。在两种情况下，在细胞质中观察到特定的基质区域。根据真菌病毒的理化性质和粒径大小，可将其分为25- 30 nm组和35- 45 nm组。（<II>）真菌传病毒：研究了由油藓属和多粘菌属传播的10种土传植物病毒。根据病毒的地理分布、病毒颗粒形态、理化性质和细胞内外观对每种病毒进行分类。因此，细长病毒可分为以下3组：（1）SBWMV、BNYVV、BNMV组，（2）BYMV、WYMV组，（3）LBVV、TSV组。将球形病毒分为3组，分别为TNV组，PSNV、MNSV组，SV组。通过比较研究和分类群，可以看出真菌传播的病毒种类繁多。少

( <I> ) Fungal parasitic viruses: Fungal viruses were screened over 600 fungal isolates 103 genus, 200 species) mainly phytopathogenic fungi. In the coures of this experiment, 41 new viruses were detected from 35 species and 7 known viruses were recognized from 6 species, suggesting that fungal viruses are wide-spread among various of these microorganisms. Direct negative staining method was shown to be the most efficient technigue to detect fungal viruses. Most of the viruses used in this study were purified from their hosts and morphological and physicochemical properties were examined. Some of them were multi component due to the differences in nucleic acid contents. Double-shell capsid structure was observed in 4 viruses and two of them posssed spikes at the 12 vertices, seemed to be taxonomically important. Serological properties of the fungal viruses were tested using virus antiserum against 7 viruses and two of them were sero-logically related with each other. Intracellular appe … More arance of each viruses were studied. Usually, viruses were recognized as both diffused and aggregated or crystalline arrays within the cytoplasm or vacuole. In two cases, specific matrix regions were observed in the cytoplasm. Fungal viruses seemed to be classified into two classes of particle diameter of 25-30nm group and 35-45nm group according to their physicochemical properties and particle sizes. ( <II> ) Fungal-borne viruses: 10 soil-borne plant viruses transmitted by Olpidium and Polymyxa were studied. Classification of each viruses were examined in regard to their geographical distribution, morphology of the virus particles, physicochemical properties and intracellular appearances. Consequently, elongated viruses were divided into following 3 groups, (1) SBWMV,BNYVV,BNMV group, (2) BYMV, WYMV group, (3) LBVV,TSV group. And spherical viruses were divided into following 3 groups, (1) TNV group, (2) PSNV,MNSV group, (3) SV group , respectively. Varieties of fungal-borne viruses were evident by this comparative study and classification groups. Less